Isolation and Identification of Bioluminescent Organism From Marine Water Sample

Bioluminescence is the emission of light, through a chemical reaction, by living organisms. Around 700 genera are found to be luminescent in whole world. Most of the organism that emit light share the chemical components involved in light production, these are referred as luciferin and luciferase. In present study, five bioluminescent bacterial strains were isolated from 3 marine water samples collected from beaches of Goa, Pondicherry, Kashid. Enrichment of bioluminescent bacteria was done in artificial sea water broth (SWB) and Luminescent Broth (LB). Purification of the bacteria was done on LA (Luminescent Agar).The isolated pure cultures were then identified by MALDI-TOF MS, where they showed similarity with two well-known bioluminescent bacterial species viz. Vibioharveyi and Vibrio parahaemolyticus.