ISOLATION AND IDENTIFICATION OF ACTINOMYCETES FROM MUNICIPAL WASTE SOIL AND THEIR SCREENING FOR ANTAGONASTIC ACTIVITY

In the present study, the soil samples were selected from municipal waste of Bilaspur city, chattisgarh. Municipal solid waste (MSW) is a rich source of lingo-cellulosic materials thus providing an intense environmental for the growth of bacteria. The actinomycetes were screened out and isolated from the pre-treated soil samples by serial dilution method on starch casein agar medium and AIA on spread plate technique. After 72 h, whitish pin-point colonies characteristics of actinomycetes. In sample 1 plate 10-1 103, 10-3 1, and 10-5 82 colonies sample 2 plate 10-1 98, 10-3 105 colonies obtained. The total 10 different isolates were selected found to be gram positive and filamentous. All isolates were later purified and subjected to biochemical tests. The purified colonies were performed in biochemical tests such as, IMViC test, catalase, oxidase, motility test, carbohydrates fermentation test, TSI test. All the isolates were subjected to a few enzymatic screening like cellulose, starch, urease and casein hydrolysis test. All the isolates were screen antagonistic activity by modified cross streak method on MHA and starch casein agar medium. The antagonistic activities were tested against E.coli ATCC strain (gram negative) and S.aureus (gram positive). All colonies show positive result except C-1 for S.aureus and all colonies show negative result for E.coli except C-3.