DEMONSTRATION OF TRANSMISSION OF ANTIBIOTIC RESISTANCE PLASMID FROM RESISTANT TO SENSITIVE STRAIN OF ESCHERICHIA COLI AND KLEBSIELLA PNEUMONIAE AT 37 ° C.

In human body numerous bacteria are present in the form of normal bacterial flora. As many of them doesn’t came in contact of antibiotics shows sensitivity at the time of contact. When the patient is in continuous contact with hospital or community acquired infection, his normal flora becomes resistant to those antibiotics which he or she is taking during treatment. Escherichia coli and Klebsiella pneumoniae are the most common hospital acquired pathogen which secretes Extended Spectrum Beta Lactamase enzyme to hydrolyze the beta lactam ring most of the antibiotics due to which it becomes resistant to that particular antibiotic. The resistant bacteria transfer its plasmid to sensitive bacteria and thus the sensitive one become resistant. So for the diagnosis of these microbes we apply Disc Diffusion Method in which strains of both sensitive and resistant bacteria is inoculated and then mixed in fixed proportion and then sensitivity test is done.