Exploring and Characterizing Cultivable Bacterial Diversity from Dumping Ground in Ambernath, India

Dumping grounds are potent site for growth and multiplication of extremophiles especially thermophile due to varying composition of carbon and nitrogen source and also high temperature which favours growth. The dumping site selected for isolation in this study contains Industrial and domestic wastes. Physicochemical characterisation of all these isolates was carried out from DSSTL Thane. In the present study, total 08 isolates with different morphotypes were obtained which were further characterised for pH, temperature and salt tolerance. Among the eight isolates, isolate CM-55 showed growth up to pH 9, Salt 4 % and able to tolerate temperature up to 950C. Isolate CM-55 was further characterized using 16SrRNA gene sequencing. Its taxonomic classification was revealed to beclosest taxonomic neighbour of Bacillus halotoleransATCC 25096(T) with 99.84% sequence similarity.